22/11-2021 - Emmanuelle Havis : Comment et pourquoi construire un tendon à partir de cellules souches adipeuses humaines ?

18 - Novembre - 2021

LES LUNDIS DE SAINT-ANTOINE

Bâtiment Kourilsky - 13h–14h

Salle des Conférences (Rez de Chaussée),

184 rue du Faubourg Saint-Antoine, Paris

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LUNDI 22 NOVEMBRE 2021

Comment et pourquoi construire un tendon à partir de cellules souches adipeuses humaines ?

Emmanuelle Havis

MCU, IBPS, Sorbonne Université

Invitée par Equipe Fève, (équipe B Fève) (bruno.feve@inserm.fr)

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Tendons are composed of a dense extracellular matrix of type I collagen fibrils that are hierarchically organized to withstand tensile forces transmitted from muscle to bone. The understanding of tendon biology lags behind that of other components of the musculoskeletal system, which prevents the development of efficient therapies for tendon repair. Our first objective is to understand how mechanical forces control tendon formation both in vivo, during chicken development and in vitro, during tendon organoid formation. For in vivo approaches, chicken embryos are subjected to pharmacological treatments leading to immobilisation and hypermotility. The consequences of these treatments are analysed for the expression of tendon markers. For in vitro studies, we are taking advantage of the FlexCell® culture system which allows the production of tendon organoids from human adipose stem cells (hASC) embedded in a collagen-gel matrix and cultured in a cylindrical mold conferring a tendon-like shape. Using this technology, we are testing the consequences of mechanical stimulations for tendon morphology, molecular signals and biomechanical properties. In addition, causes of tendinopathies are multiple and include excess load, metabolic disorders, ageing and drugs. Tendon atrophy is observed after the administration of high doses of different classes of molecules such as corticoids or quinolone antibiotics. Our second objective is to develop advanced relevant tendon organoid models to evaluate the side effects of novel molecules, not only on the classical target organs but also on other tissues, such as tendon.

 

Afin d'assister à cette conférence vous pourrez vous connecter sur le lien zoom suivant :

https://us02web.zoom.us/j/81762143062?pwd=VndrcWc3elNwSWhnb3pWaWljQnZVUT09 ID de réunion : 817 6214 3062 Code secret : 061417

Hôpital St-Antoine

Bâtiment Kourilsky
34 rue Crozatier
75012 PARIS

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