• Vendredi, 18 Septembre 2020 - 22:06:05

Postprandial bile acid levels in intestine and plasma reveal altered biliary circulation in chronic pancreatitis patients (Équipe Seksik/Sokol)

11 - Septembre - 2018

Lydie Humbert, Dominique Rainteau, Noshine Tuvignon, Claude Wolf, Philippe Seksik, René Laugier, Frédéric Carriere

J Lipid Res. 2018;59(11):2202-13

Bile acid (BA) secretion and circulation in chronic pancreatitis (CP) patients with exocrine pancreatic insufficiency (EPI) were investigated by simultaneously measuring postprandial levels of individual BAs in duodenal contents and blood plasma using LC-MS/MS. CP patients and healthy volunteers (HVs) were intubated with gastric and duodenal tubes prior to the administration of a test meal and continuous aspiration of duodenal contents. Pancreatic lipase outputs in CP patients were very low (0.7 +/- 0.2 mg) versus HVs (116.7 +/- 68.1 mg; P < 0.005), thus confirming the severity of EPI. Duodenal BA outputs were reduced in CP patients (1.00 +/- 0.89 mmol; 0.47 +/- 0.42 g) versus HVs (5.52 +/- 4.53 mmol; 2.62 +/- 2.14 g; P < 0.15). Primary to secondary BA ratio was considerably higher in CP patients (38.09 +/- 48.1) than HVs (4.15 +/- 2.37; P < 0.15), indicating an impaired transformation of BAs by gut microbiota. BA concentrations were found below the critical micellar concentration in CP patients, while a high BA concentration peak corresponding to gallbladder emptying was evidenced in HVs. Conversely, BA plasma concentration was increased in CP patients versus HVs suggesting a cholangiohepatic shunt of BA secretion. Alterations of BA circulation and levels may result from the main biliary duct stenosis observed in these CP patients and may aggravate the consequences of EPI on lipid malabsorption.

2018 J Lipd Res - Humbert (fig).jpg

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